Anaxyrus americanus

American toad

Maximum lifespan

36 yr

AnAge record

mtDNA size

17,328 bp

RefSeq NC_047224.1

GC content

37.59%

Whole genome

Non-coding

1,997 bp

D-loop 1,916 bp

Where American toad sits among 2,335 species

Whole-genome GC content against maximum lifespan. American toad has the 312th longest lifespan of them.

This species
Other amphibians
Other classes

Plot other mtDNA features with this species highlighted

Base composition

Whole genome17,328 bp

GC skew (G − C) / (G + C): −0.248
AT skew (A − T) / (A + T): −0.038

Life history

Maximum lifespan: 36 years
Adult weight: 21.8 g
Basal metabolic rate: 0.008 W
Body temperature: 14.9 °C
Female sexual maturity: 2.5 years
Male sexual maturity: 2.5 years
Gestation or incubation: 8 days
Litter or clutch size: 6,000

Lifespan from AnAge · AnAge record · other traits from AnAge build 15 (HAGRID 00036)

About this record

Lifespan source: AnAge build 15
Based on: an animal in captivity
Data quality: acceptable, medium sample
Licence: Free to use, subject to the Human Ageing Genomic Resources conditions of use
Status: New in build 2
In the database since: build 2 (2026-11-13)

Every change against the 2015 build is in the changelog download.

Composition by region

Reference (L) strand as deposited in RefSeq

Whole genome17,328 bp

Protein-coding11,265 bp

12S rRNA934 bp

16S rRNA1,602 bp

All tRNA1,530 bp

D-loop1,916 bp

Genes and regions

Select a column to sort; select a gene to compare it across species

Base composition of Anaxyrus americanus genes

ND1NADH dehydrogenase subunit 1	961 bp	28.5%	33.4%	12.3%	25.8%	38.09%	−0.355	−0.079
ND2NADH dehydrogenase subunit 2	1,033 bp	29.1%	32.9%	10.4%	27.6%	37.95%	−0.454	−0.061
COX1Cytochrome c oxidase subunit I	1,542 bp	26.1%	34.6%	16.7%	22.6%	39.30%	−0.152	−0.139
COX2Cytochrome c oxidase subunit II	685 bp	30.4%	30.9%	15.8%	22.9%	38.69%	−0.185	−0.010
ATP8ATP synthase F0 subunit 8	165 bp	31.5%	38.2%	9.7%	20.6%	30.30%	−0.360	−0.096
ATP6ATP synthase F0 subunit 6	684 bp	27.2%	35.2%	11.0%	26.6%	37.57%	−0.416	−0.129
COX3Cytochrome c oxidase subunit III	784 bp	26.8%	33.8%	16.5%	23.0%	39.41%	−0.165	−0.116
ND3NADH dehydrogenase subunit 3	340 bp	24.4%	39.1%	14.7%	21.8%	36.47%	−0.194	−0.231
ND4LNADH dehydrogenase subunit 4L	300 bp	27.3%	33.7%	12.0%	27.0%	39.00%	−0.385	−0.104
ND4NADH dehydrogenase subunit 4	1,365 bp	27.2%	35.2%	12.4%	25.2%	37.58%	−0.341	−0.129
ND5NADH dehydrogenase subunit 5	1,789 bp	30.2%	34.3%	12.2%	23.3%	35.50%	−0.313	−0.062
ND6NADH dehydrogenase subunit 6	495 bp	38.0%	20.8%	9.5%	31.7%	41.21%	−0.539	+0.292
CYTBCytochrome b	1,143 bp	25.7%	34.7%	13.5%	26.1%	39.55%	−0.319	−0.149
12S rRNA12S ribosomal RNA	934 bp	31.2%	24.5%	20.0%	24.3%	44.33%	−0.097	+0.119
16S rRNA16S ribosomal RNA	1,602 bp	35.5%	28.1%	16.8%	19.7%	36.45%	−0.079	+0.116
D-loopControl region (D-loop)	1,916 bp	34.1%	36.2%	11.8%	17.9%	29.75%	−0.204	−0.030

Codon usage

Counts per codon, darker is more frequent

Gene

3,760 codons in all protein-coding genes. Grouped with the Vertebrate mitochondrial code (NCBI table 2).

Phe F6.8%

UUU202

UUC55

Leu L16.2%

UUA176

UUG23

CUU182

CUC80

CUA125

CUG24

Ile I9.0%

AUU281

AUC57

Met M4.6%

AUA131

AUG42

Val V4.9%

GUU83

GUC25

GUA63

GUG14

Ser S8.0%

UCU93

UCC50

UCA96

UCG13

AGU24

AGC24

Pro P5.4%

CCU61

CCC38

CCA95

CCG8

Thr T7.5%

ACU78

ACC77

ACA114

ACG12

Ala A8.1%

GCU92

GCC111

GCA92

GCG8

Tyr Y2.9%

UAU58

UAC50

His H2.3%

CAU38

CAC47

Gln Q2.3%

CAA83

CAG5

Asn N3.6%

AAU67

AAC68

Lys K2.3%

AAA73

AAG12

Asp D2.0%

GAU37

GAC37

Glu E2.5%

GAA71

GAG22

Cys C0.8%

UGU12

UGC19

Trp W3.0%

UGA102

UGG9

Arg R1.9%

CGU9

CGC16

CGA43

CGG3

Gly G5.9%

GGU39

GGC47

GGA103

GGG34

Stop 0.2%

UAA5

UAG0

AGA1

AGG1

Genome map

38 annotated features in NC_047224.1

Hover, tap or tab to a feature to see its position, length, strand and GC content. Genes and rRNAs open their own pages.

Protein-coding13
rRNA2
tRNA22
D-loop1

Outer ring: + strand, arrows run clockwise. Inner ring: − strand.
Middle ring: GC% of each gene against the whole genome (37.6%, dashed): out is GC-richer, in is AT-richer (full height = 6 points).

All 38 features as a table

Annotated features of Anaxyrus americanus (NC_047224.1)
Feature	Type	Start	End	Length	Strand	GC
tRNA-Leu (CUN)	Transfer RNA	1	72	72 bp	+	–
tRNA-Thr	Transfer RNA	73	144	72 bp	+	–
tRNA-Pro	Transfer RNA	144	212	69 bp	−	–
tRNA-Phe	Transfer RNA	212	279	68 bp	+	–
12S rRNA	Ribosomal RNA	280	1,213	934 bp	+	44.3%
tRNA-Val	Transfer RNA	1,214	1,282	69 bp	+	–
16S rRNA	Ribosomal RNA	1,283	2,884	1,602 bp	+	36.5%
tRNA-Leu (UUR)	Transfer RNA	2,885	2,957	73 bp	+	–
ND1	Protein-coding gene	2,958	3,918	961 bp	+	38.1%
tRNA-Ile	Transfer RNA	3,919	3,989	71 bp	+	–
tRNA-Gln	Transfer RNA	3,989	4,059	71 bp	−	–
tRNA-Met	Transfer RNA	4,059	4,127	69 bp	+	–
ND2	Protein-coding gene	4,128	5,160	1,033 bp	+	38.0%
tRNA-Trp	Transfer RNA	5,161	5,230	70 bp	+	–
tRNA-Ala	Transfer RNA	5,232	5,300	69 bp	−	–
tRNA-Asn	Transfer RNA	5,302	5,374	73 bp	−	–
tRNA-Cys	Transfer RNA	5,401	5,464	64 bp	−	–
tRNA-Tyr	Transfer RNA	5,465	5,534	70 bp	−	–
COX1	Protein-coding gene	5,539	7,080	1,542 bp	+	39.3%
tRNA-Ser (UCN)	Transfer RNA	7,081	7,151	71 bp	−	–
tRNA-Asp	Transfer RNA	7,156	7,224	69 bp	+	–
COX2	Protein-coding gene	7,226	7,910	685 bp	+	38.7%
tRNA-Lys	Transfer RNA	7,911	7,982	72 bp	+	–
ATP8	Protein-coding gene	7,984	8,148	165 bp	+	30.3%
ATP6	Protein-coding gene	8,139	8,822	684 bp	+	37.6%
COX3	Protein-coding gene	8,822	9,605	784 bp	+	39.4%
tRNA-Gly	Transfer RNA	9,606	9,674	69 bp	+	–
ND3	Protein-coding gene	9,675	10,014	340 bp	+	36.5%
tRNA-Arg	Transfer RNA	10,015	10,083	69 bp	+	–
ND4L	Protein-coding gene	10,084	10,383	300 bp	+	39.0%
ND4	Protein-coding gene	10,377	11,741	1,365 bp	+	37.6%
tRNA-His	Transfer RNA	11,742	11,810	69 bp	+	–
tRNA-Ser (AGY)	Transfer RNA	11,811	11,877	67 bp	+	–
ND5	Protein-coding gene	11,915	13,703	1,789 bp	+	35.5%
ND6	Protein-coding gene	13,701	14,195	495 bp	−	41.2%
tRNA-Glu	Transfer RNA	14,196	14,263	68 bp	−	–
CYTB	Protein-coding gene	14,269	15,411	1,143 bp	+	39.6%
D-loop	Control region	15,413	17,328	1,916 bp	–	29.8%