Pelophylax nigromaculatus

Black-spotted frog

Maximum lifespan

15 yr

AnAge record

mtDNA size

17,804 bp

RefSeq NC_002805.1

GC content

43.07%

Whole genome

Non-coding

2,493 bp

D-loop 2,425 bp

Where Black-spotted frog sits among 2,335 species

Whole-genome GC content against maximum lifespan. Black-spotted frog has the 1,217th longest lifespan of them.

This species
Other amphibians
Other classes

Plot other mtDNA features with this species highlighted

Base composition

Whole genome17,804 bp

GC skew (G − C) / (G + C): −0.303
AT skew (A − T) / (A + T): −0.040

Life history

Maximum lifespan: 15 years
Female sexual maturity: 2 years
Male sexual maturity: 2 years
Litter or clutch size: 2,800

Lifespan from AnAge · AnAge record · other traits from AnAge Build 15 (HAGRID 00117)

About this record

Lifespan source: AnAge build 15
Based on: an animal in the wild
Data quality: acceptable, medium sample
Licence: Free to use, subject to the Human Ageing Genomic Resources conditions of use
Status: Renamed in build 2
In the database since: build 1 (2015-07-01)
Last changed: build 2 (2026-11-13)
Earlier name: Rana nigromaculata

Every change against the 2015 build is in the changelog download.

Composition by region

Reference (L) strand as deposited in RefSeq

Whole genome17,804 bp

Protein-coding11,261 bp

12S rRNA933 bp

16S rRNA1,588 bp

All tRNA1,532 bp

D-loop2,425 bp

Genes and regions

Select a column to sort; select a gene to compare it across species

Base composition of Pelophylax nigromaculatus genes

ND1NADH dehydrogenase subunit 1	961 bp	22.9%	31.6%	12.6%	32.9%	45.47%	−0.446	−0.160
ND2NADH dehydrogenase subunit 2	1,038 bp	27.0%	30.2%	10.6%	32.3%	42.87%	−0.506	−0.056
COX1Cytochrome c oxidase subunit I	1,539 bp	24.5%	31.0%	17.3%	27.2%	44.51%	−0.223	−0.117
COX2Cytochrome c oxidase subunit II	688 bp	29.7%	28.1%	15.3%	27.0%	42.30%	−0.278	+0.028
ATP8ATP synthase F0 subunit 8	165 bp	35.2%	26.7%	9.7%	28.5%	38.18%	−0.492	+0.137
ATP6ATP synthase F0 subunit 6	682 bp	24.9%	30.9%	11.4%	32.7%	44.14%	−0.482	−0.108
COX3Cytochrome c oxidase subunit III	784 bp	23.3%	28.7%	16.7%	31.3%	47.96%	−0.303	−0.103
ND3NADH dehydrogenase subunit 3	340 bp	21.2%	31.8%	14.1%	32.9%	47.06%	−0.400	−0.200
ND4LNADH dehydrogenase subunit 4L	285 bp	24.6%	31.9%	15.1%	28.4%	43.51%	−0.306	−0.130
ND4NADH dehydrogenase subunit 4	1,360 bp	25.9%	31.8%	12.9%	29.5%	42.35%	−0.392	−0.102
ND5NADH dehydrogenase subunit 5	1,795 bp	26.0%	31.0%	13.8%	29.1%	42.95%	−0.357	−0.088
ND6NADH dehydrogenase subunit 6	501 bp	34.5%	17.6%	13.6%	34.3%	47.90%	−0.433	+0.326
CYTBCytochrome b	1,143 bp	23.4%	28.7%	14.5%	33.3%	47.86%	−0.393	−0.101
12S rRNA12S ribosomal RNA	933 bp	29.8%	22.7%	20.8%	26.7%	47.48%	−0.124	+0.135
16S rRNA16S ribosomal RNA	1,588 bp	32.9%	24.8%	18.1%	24.2%	42.32%	−0.143	+0.140
D-loopControl region (D-loop)	2,425 bp	29.0%	36.4%	13.0%	21.6%	34.60%	−0.249	−0.113

Codon usage

Counts per codon, darker is more frequent

Gene

3,758 codons in all protein-coding genes. Grouped with the Vertebrate mitochondrial code (NCBI table 2).

Phe F7.2%

UUU144

UUC125

Leu L16.7%

UUA98

UUG15

CUU148

CUC167

CUA140

CUG58

Ile I8.1%

AUU206

AUC98

Met M4.1%

AUA98

AUG55

Val V5.3%

GUU70

GUC58

GUA60

GUG12

Ser S7.2%

UCU65

UCC87

UCA61

UCG9

AGU12

AGC36

Pro P5.5%

CCU42

CCC72

CCA82

CCG9

Thr T7.6%

ACU64

ACC115

ACA104

ACG3

Ala A8.7%

GCU61

GCC182

GCA72

GCG11

Tyr Y3.0%

UAU53

UAC58

His H2.7%

CAU29

CAC72

Gln Q2.3%

CAA78

CAG10

Asn N3.4%

AAU59

AAC67

Lys K2.3%

AAA75

AAG12

Asp D2.0%

GAU28

GAC48

Glu E2.3%

GAA75

GAG13

Cys C0.8%

UGU11

UGC19

Trp W2.8%

UGA88

UGG19

Arg R1.9%

CGU11

CGC24

CGA30

CGG7

Gly G6.0%

GGU37

GGC91

GGA57

GGG42

Stop 0.2%

UAA3

UAG2

AGA1

AGG0

Genome map

38 annotated features in NC_002805.1

Hover, tap or tab to a feature to see its position, length, strand and GC content. Genes and rRNAs open their own pages.

Protein-coding13
rRNA2
tRNA22
D-loop1

Outer ring: + strand, arrows run clockwise. Inner ring: − strand.
Middle ring: GC% of each gene against the whole genome (43.1%, dashed): out is GC-richer, in is AT-richer (full height = 6 points).

All 38 features as a table

Annotated features of Pelophylax nigromaculatus (NC_002805.1)
Feature	Type	Start	End	Length	Strand	GC
D-loop	Control region	1	2,425	2,425 bp	–	34.6%
tRNA-Leu (CUN)	Transfer RNA	2,426	2,497	72 bp	+	–
tRNA-Thr	Transfer RNA	2,500	2,569	70 bp	+	–
tRNA-Pro	Transfer RNA	2,570	2,638	69 bp	−	–
tRNA-Phe	Transfer RNA	2,640	2,709	70 bp	+	–
12S rRNA	Ribosomal RNA	2,710	3,642	933 bp	+	47.5%
tRNA-Val	Transfer RNA	3,642	3,710	69 bp	+	–
16S rRNA	Ribosomal RNA	3,711	5,298	1,588 bp	+	42.3%
tRNA-Leu (UUR)	Transfer RNA	5,300	5,373	74 bp	+	–
ND1	Protein-coding gene	5,375	6,335	961 bp	+	45.5%
tRNA-Ile	Transfer RNA	6,336	6,406	71 bp	+	–
tRNA-Gln	Transfer RNA	6,407	6,477	71 bp	−	–
tRNA-Met	Transfer RNA	6,477	6,545	69 bp	+	–
ND2	Protein-coding gene	6,546	7,583	1,038 bp	+	42.9%
tRNA-Trp	Transfer RNA	7,582	7,651	70 bp	+	–
tRNA-Ala	Transfer RNA	7,652	7,721	70 bp	−	–
tRNA-Asn	Transfer RNA	7,722	7,794	73 bp	−	–
tRNA-Cys	Transfer RNA	7,822	7,888	67 bp	−	–
tRNA-Tyr	Transfer RNA	7,888	7,954	67 bp	−	–
COX1	Protein-coding gene	7,959	9,497	1,539 bp	+	44.5%
tRNA-Ser (UCN)	Transfer RNA	9,500	9,570	71 bp	−	–
tRNA-Asp	Transfer RNA	9,573	9,641	69 bp	+	–
COX2	Protein-coding gene	9,642	10,329	688 bp	+	42.3%
tRNA-Lys	Transfer RNA	10,330	10,398	69 bp	+	–
ATP8	Protein-coding gene	10,399	10,563	165 bp	+	38.2%
ATP6	Protein-coding gene	10,554	11,235	682 bp	+	44.1%
COX3	Protein-coding gene	11,236	12,019	784 bp	+	48.0%
tRNA-Gly	Transfer RNA	12,020	12,088	69 bp	+	–
ND3	Protein-coding gene	12,089	12,428	340 bp	+	47.1%
tRNA-Arg	Transfer RNA	12,429	12,497	69 bp	+	–
ND4L	Protein-coding gene	12,498	12,782	285 bp	+	43.5%
ND4	Protein-coding gene	12,776	14,135	1,360 bp	+	42.4%
tRNA-His	Transfer RNA	14,136	14,204	69 bp	+	–
tRNA-Ser (AGY)	Transfer RNA	14,205	14,271	67 bp	+	–
ND5	Protein-coding gene	14,297	16,091	1,795 bp	+	43.0%
ND6	Protein-coding gene	16,089	16,589	501 bp	−	47.9%
tRNA-Glu	Transfer RNA	16,591	16,659	69 bp	−	–
CYTB	Protein-coding gene	16,662	17,804	1,143 bp	+	47.9%